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SUMMARY:Short Talk 1\, Veronika Nesverova - Structural insights into AQP2 
 targeting to multivesicular bodies
DTSTART;VALUE=DATE-TIME:20191009T115500Z
DTEND;VALUE=DATE-TIME:20191009T121500Z
DTSTAMP;VALUE=DATE-TIME:20260525T223624Z
UID:indico-contribution-753@lindico453.srv.lu.se
DESCRIPTION:Speakers: Veronika Nesverova (Lund University)\nThe multivesic
 ular sorting machinery is a crucial mechanism for targeting membrane prote
 ins\nfor recycling or degradation. The lysosomal trafficking regulator-int
 eracting protein 5 (LIP5)\nwhich coordinates the action of this machinery 
 is also known to bind directly to the membrane\nprotein cargo. In case of 
 aquaporin 2 (AQP2) the binding of LIP5 during the endocytic pathway\nin ki
 dney collecting duct cells ensures an effective regulation of urine volume
  [1].\nIn our group\, we have previously studied the role of AQP2 phosphor
 ylation in AQP2-LIP5\ninteraction [2]. Currently we are focusing on elucid
 ating the structural details of the complex\nin order to better understand
  how membrane proteins are delivered to the multivesicular\nbodies. We hav
 e constructed alanine mutants of single residues in the proposed binding s
 ites\nof both AQP2 and LIP5. Studying the binding affinity of these mutant
 s using fluorescence\nquenching helps us understand which residues are dir
 ectly involved in the binding.\nFurther\, AQP2 was successfully incorporat
 ed into MSP-based nanodiscs and negative stain\nelectron microscopy confir
 med homogeneous state of the particles. We have collected high\nresolution
  images on Titan Krios and are currently processing the data.\n\n[1] B. W.
  M. Van Balkom\, M. Boone\, G. Hendriks\, E. Kamsteeg\, J. H. Robben\, H. 
 C. Stronks\, A.\nVan Der Voorde\, and F. Van Herp\, “LIP5 Interacts with
  Aquaporin 2 and Facilitates Its\nLysosomal Degradation\,” pp. 990–100
 1\, 2009.\n[2] J. V. Roche\, S. Survery\, S. Kreida\, V. Nesverova\, H. Am
 pah-Korsah\, M. Gourdon\, P. M. T.\nDeen\, and S. Törnroth-Horsefield\, 
 “Phosphorylation of human aquaporin 2 (AQP2)\nallosterically controls it
 s interaction with the lysosomal trafficking protein LIP5\,” J. Biol.\nC
 hem.\, vol. 292\, no. 35\, pp. 14636–14648\, 2017.\n\nhttps://lindico453
 .srv.lu.se/event/125/contributions/753/
LOCATION:Kulturen Auditorium
URL:https://lindico453.srv.lu.se/event/125/contributions/753/
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