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SUMMARY:Keynote 2\, Prof. Yifan Cheng: Single particle cryo-EM of membrane
  proteins
DTSTART;VALUE=DATE-TIME:20191009T123500Z
DTEND;VALUE=DATE-TIME:20191009T131500Z
DTSTAMP;VALUE=DATE-TIME:20260526T034225Z
UID:indico-contribution-755@lindico453.srv.lu.se
DESCRIPTION:Speakers: Yifan Cheng (UC San Francisco)\nWith the technologic
 al breakthroughs in the past few years\, single particle cryo-electron mic
 roscopy (cryo-EM) has enabled rapid progresses in structure determination 
 of integral membrane proteins\, particularly ion channels. The pace of str
 ucture determination of integral membrane proteins by single particle cryo
 -EM is unprecedented in structural biology. With such a rapid progress\, i
 t is also very critical to interpretation of cryo-EM density maps carefull
 y to ensure that interpretation is data-driven. I will discuss some practi
 cal examples to demonstrate the significance of careful interpretations of
  single particle cryo-EM density maps. \n\nFurthermore\, as a prominent ex
 ample in structural biology of membrane proteins\, structural studies of t
 ransient receptor potential (TRP) channel superfamily demonstrated nicely 
 how technological breakthroughs impacts scientific discoveries. As an exam
 ple of our recent studies of TRP channels by single particle cryo-EM\, TRP
 V5 (transient receptor potential vanilloid 5) represents a unique calcium-
 selective TRP channel essential for calcium homeostasis. Unlike other TRPV
  channels\, TRPV5 and its close homolog\, TRPV6\, do not exhibit thermosen
 sitivity or ligand-dependent activation but are constitutively open at phy
 siological membrane potentials and modulated by calmodulin (CaM) in a calc
 ium-dependent manner. Structural studies of truncated and full-length TRPV
 5 in lipid nanodiscs\, as well as of a TRPV5 W583A mutant and TRPV5 in com
 plex with CaM provide novel insights to the mechanism of calcium regulatio
 n and reveal a flexible stoichiometry of CaM binding to TRPV5.\n\nhttps://
 lindico453.srv.lu.se/event/125/contributions/755/
LOCATION:Kulturen Auditorium
URL:https://lindico453.srv.lu.se/event/125/contributions/755/
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