BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//CERN//INDICO//EN
BEGIN:VEVENT
SUMMARY:Short Talk 5\, Ronnie Berntsson - Structural insight into Gram-pos
 itive Type 4 Secretion Systems
DTSTART;VALUE=DATE-TIME:20191010T074000Z
DTEND;VALUE=DATE-TIME:20191010T080000Z
DTSTAMP;VALUE=DATE-TIME:20260526T072136Z
UID:indico-contribution-761@lindico453.srv.lu.se
DESCRIPTION:Speakers: Ronnie Berntsson (Umeå University)\nMultidrug resis
 tance in bacteria\, originating from conjugative gene transfer\, is an inc
 reasingly common problem\nin today’s world. The majority of bacteria tha
 t causes hospital infections are of gram-positive origin\,\nbut so far ver
 y little is known about their conjugation systems. To remedy this\, we aim
  to determine the\nmolecular structure and function of conjugation complex
 es belonging to Type IV Secretion Systems (T4SSs)\nfrom gram-positive bact
 eria. This will lead to a deeper insight into one of the main processes re
 sponsible for\nhorizontal gene transfer events\, including the spread of a
 ntibiotic resistance genes in bacteria.\nWe study the proteins involved in
  forming the T4SS biochemically\, structurally and biophysically. Since gr
 ampositive\nT4SSs are very dissimilar from their gram-negative counterpart
 s\, little can be deduced from the few\ngram-negative systems so far studi
 ed. Furthermore\, they occur in a number of pathogens\, such as enterococc
 i\,\nstreptococci and staphylococci. Another aspect that makes gram-positi
 ve T4SSs interesting is that they are\nused to efficiently transfer not on
 ly antibiotic resistance\, but also virulence factors.\nThese megadalton s
 ized systems are built up by i) extracellular adhesion proteins\, ii) memb
 rane channel proteins\nand iii) intracellular DNA processing proteins. Her
 e\, I will present our current understanding of the\nT4SS originating from
  the conjugative plasmid pCF10 of Enterococcus faecalis. Our work on this 
 system\ncombines molecular biology\, biochemistry\, X-ray crystallography 
 and Electron Microscopy. This has so far\nallowed us to determine structur
 es and understand some of the functions of the adhesion proteins as well a
 s\npart of the DNA processing proteins\, which will highlight both major d
 ifferences and similarities between the\ngram-positive and gram-negative s
 ystems.\n\nhttps://lindico453.srv.lu.se/event/125/contributions/761/
LOCATION:Kulturen Auditorium
URL:https://lindico453.srv.lu.se/event/125/contributions/761/
END:VEVENT
END:VCALENDAR