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SUMMARY:Keynote 6\, Dr. Andrey Gruzinov: Small-angle X-ray scattering (SAX
 S) for integrative structural biology
DTSTART;VALUE=DATE-TIME:20191010T093000Z
DTEND;VALUE=DATE-TIME:20191010T101000Z
DTSTAMP;VALUE=DATE-TIME:20260525T195543Z
UID:indico-contribution-764@lindico453.srv.lu.se
DESCRIPTION:Speakers: Andrey Gruzinov (EMBL c/o DESY\, Hamburg\, Germany)\
 nSmall-angle X-ray scattering (SAXS) is a powerful method in the studies o
 f solutions of biological macromolecules and nanostructured systems [1] al
 lowing one to analyze the structure of native particles and complexes and 
 to rapidly assess structural changes in response to variations in external
  conditions. Dedicated high brilliance synchrotron beamlines and novel dat
 a analysis methods [2] significantly enhanced resolution and reliability o
 f the structural models provided by SAXS. Very important is the ability of
  SAXS to quantitatively characterize complicated systems and mixtures in n
 ative environments and to see the biomolecules in action by rapidly observ
 ing responses to changing physical and chemical conditions (e.g. upon pH o
 r temperature changes\, ligand binding etc). \nGiven the limited informati
 on content in the scattering data\, robust data analysis and modelling met
 hods are of major importance for broad applications of solution SAXS in bi
 ology. To reduce the ambiguity of interpretation\, SAXS is often combined 
 with other structural methods like crystallography\, NMR and electron micr
 oscopy\, and also with computational\, biophysical and biochemical techniq
 ues to build hybrid models. In classical applications\, SAXS generally yie
 lds low resolution quaternary structure but\, very importantly\, the metho
 d can also help to analyze equilibrium mixtures and to visualize flexible 
 portions of the structures\, not seen by the high resolution methods. \nIn
  the present talk\, modern methods for SAXS data analysis will be presente
 d and illustrated by applications to characterize structures and conformat
 ional transitions of biological macromolecules in solution. Recent develop
 ments including\, in particular\, in-line chromatography approaches [3] wi
 ll be elucidated and perspectives of the synergistic use of SAXS for integ
 rative modeling utilizing complementary methods will be discussed.\n\nhttp
 s://lindico453.srv.lu.se/event/125/contributions/764/
LOCATION:Kulturen Auditorium
URL:https://lindico453.srv.lu.se/event/125/contributions/764/
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