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SUMMARY:Short Talk 7\, Ryan Oliver - Integrative structural biology with c
 omplementary experimental methods to describe viral capsid protein self-as
 sembly
DTSTART;VALUE=DATE-TIME:20191010T101000Z
DTEND;VALUE=DATE-TIME:20191010T103000Z
DTSTAMP;VALUE=DATE-TIME:20260526T101413Z
UID:indico-contribution-765@lindico453.srv.lu.se
DESCRIPTION:Speakers: Ryan Oliver (Dept of Biochemistry and Structural Bio
 logy)\nThe genetic code of viruses\, RNA or DNA\, are typically protected 
 in an icosahedral capsid\, which is primarily\nassembled from over a hundr
 ed subunits of the same protein in a spontaneous self-assembly process. Si
 milar\nhighly efficient assembly processes are ubiquitous in biological sy
 stems\; viral capsids present a unique platform\nto exploit for therapeuti
 c advances in the targeted cellular delivery of cargo packaged within the 
 capsid.\nOur research aims to provide a more detailed understanding of how
  this precise viral capsid protein assembly\nprocess occurs from a pool of
  single building blocks\, and additionally the effect and organization of 
 nucleic\nacid present during assembly. Here\, we present results from smal
 l-angle neutron scattering experiments using\ncontrast variation to reveal
  the final assembled structural organization of both the protein and nucle
 ic\nacid components from recombinant Hepatitis B virus (HBV) capsid protei
 n and a synthetically prepared RNA\ncontaining the capsid protein binding 
 domain. These data revealed that RNA was localized along the inner capsid\
 nsurface. Time-resolved small-angle x-ray scattering (SAXS) experiments we
 re also used to determine the\nstructure during HBV capsid assembly in the
  presence and absence of RNA. We employed Bayesian statisticsbased\ncomput
 ational methods to extract kinetic parameters of assembly and the overall 
 size and shape of the\ndominant structural intermediates from the SAXS dat
 a. Additional single-particle cryoEM reconstructions are\nprovided to asse
 ss the effect of RNA on the resulting assembled capsid structure. The comb
 ination of timeresolved\nscattering data\, Bayesian statistics\, and cryoE
 M structural analysis\, provides a framework which not\nonly describes the
  viral self-assembly process\, but can be extended to other hierarchical a
 ssemblies in biology.\n\nhttps://lindico453.srv.lu.se/event/125/contributi
 ons/765/
LOCATION:Kulturen Auditorium
URL:https://lindico453.srv.lu.se/event/125/contributions/765/
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