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SUMMARY:Short Talk 9\, Annika Söderholm - Structure and substrate specifi
 city of the tryptophan biosynthesis enzyme IGPS from Pseudomonas aeruginos
 a
DTSTART;VALUE=DATE-TIME:20191010T143000Z
DTEND;VALUE=DATE-TIME:20191010T145000Z
DTSTAMP;VALUE=DATE-TIME:20260525T195543Z
UID:indico-contribution-768@lindico453.srv.lu.se
DESCRIPTION:Speakers: Annika Söderholm (Uppsala university)\nIn bacteria\
 , tryptophan synthesis is performed by the enzymes encoded in the trp oper
 on. The product of the\ntrpC gene\, indole-3-glycerol phosphate synthase (
 IGPS) catalyzes the indole-forming reaction of tryptophan\nsynthesis. The 
 reaction mechanism includes a decarboxylation step of the substrate 1-(o-c
 arboxyphenylamino)\n1-deoxyribulose 5-phosphate (CdRP). The decarboxylatio
 n has been assumed to constitute an essential step\nof the mechanism since
  no activity with the decarboxylated variant of the substrate\, phenylamin
 odeoxyribulosephosphate\n(PAdRP)\, was observed in an early study on IGPS 
 from Escherichia coli (Smith and Yanofsky\,\n1962).\nIn this study\, we de
 monstrate enzyme-catalyzed formation of the native product IGP from decarb
 oxylated\nsubstrate PAdRP using IGPS from Pseudomonas aeruginosa. Moreover
 \, the crystal structure of P. aeruginosa\nIGPS in complex with a substrat
 e analogue was solved to 2.1 Å resolution. By structural comparison to E.
 coli\nIGPS (Wilmanns et al.\, 1992)\, we provide structure-based hypothese
 s on the difference in substrate specificity\nbetween the E.coli and P. ae
 ruginosa homologs.\n\nReferences:\nSmith\, B. O. H. and Yanofsky\, C. (196
 2) ‘Enzymes Involved in the Biosynthesis of Tryptophan’\, Methods Enzy
 mol.\,\n5\, pp. 794–806.\nWilmanns\, M. et al. (1992) ‘Three-dimension
 al structure of the bifunctional enzyme phosphoribosylanthranilate\nisomer
 ase: Indoleglycerolphosphate synthase from Escherichia coli refined at 2.0
  Å resolution’\, Journal\nof Molecular Biology\, 223(2)\, pp. 477–507
 .\n\nhttps://lindico453.srv.lu.se/event/125/contributions/768/
LOCATION:Kulturen Auditorium
URL:https://lindico453.srv.lu.se/event/125/contributions/768/
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