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SUMMARY:Keynote 8\, Prof. Henning Tidow: Structural studies of integral me
 mbrane proteins using stealth carrier nanodiscs
DTSTART;VALUE=DATE-TIME:20191010T145000Z
DTEND;VALUE=DATE-TIME:20191010T153000Z
DTSTAMP;VALUE=DATE-TIME:20260526T203227Z
UID:indico-contribution-769@lindico453.srv.lu.se
DESCRIPTION:Speakers: Henning Tidow (Hamburg University)\nStructural studi
 es of integral membrane proteins (IMPs) are challenging\, as many of them 
 are inactive or insoluble in the absence of a lipid environment. We pionee
 red an approach making use of fractionally deuterium labelled ‘stealth c
 arrier’ nanodiscs that are effectively invisible to low-resolution neutr
 on diffraction and enable structural studies of IMPs in a lipidic native-l
 ike solution environment. We show the potential of the method in a joint s
 mall-angle neutron scattering (SANS) and X-ray scattering (SAXS) study of 
 the ATP-binding cassette (ABC) transporter protein MsbA solubilized in the
  stealth nanodiscs. The data allow for a direct observation of the signal 
 from the solubilized protein without contribution from the surrounding lip
 id nanodisc. Not only the overall shape but also differences between confo
 rmational states of MsbA can be reliably detected from the scattering data
 \, demonstrating the sensitivity of the approach and its general applicabi
 lity to structural studies of IMPs. In a follow-up project\, we could also
  apply this method to investigate the structural basis for the activation 
 of an essential Ca2+-pump by its regulator calmodulin.\nThis methodology c
 an be applied to other classes of integral membrane proteins and paves the
  way for low-resolution structure determination of IMPs in solution using 
 both ab initio and rigid body analysis approaches.\n\nhttps://lindico453.s
 rv.lu.se/event/125/contributions/769/
LOCATION:Kulturen Auditorium
URL:https://lindico453.srv.lu.se/event/125/contributions/769/
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