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SUMMARY:Short Talk 12\, Magnus Andersson - Tracking Ca2+ ATPase Intermedia
 tes in Real-Time by X-ray Solution Scattering
DTSTART;VALUE=DATE-TIME:20191011T085000Z
DTEND;VALUE=DATE-TIME:20191011T091000Z
DTSTAMP;VALUE=DATE-TIME:20260525T195543Z
UID:indico-contribution-773@lindico453.srv.lu.se
DESCRIPTION:Speakers: Magnus Andersson (Umeå University)\nSarco/endoplasm
 ic reticulum Ca2+ ATPase (SERCA) transporters regulate calcium signaling b
 y active calcium\nion reuptake to internal stores. Several of the structur
 al transitions associated with transport have been characterized\nby X-ray
  crystallography\, but critical intermediates of the inward-outward switch
 ing are missing.\nWe combined time-resolved X-ray solution scattering (TR-
 XSS) experiments and molecular dynamics (MD)\nsimulations for real-time tr
 acking of concerted SERCA reaction-cycle dynamics in the native membrane. 
 The\nTR-XSS pre-pulse model differed in the domain arrangement compared to
  Ca2E1 crystal structures. A 1.5\nms intermediate showed closure of the cy
 tosolic domains typical of Ca2+- and ATP-bound E1 states. A subsequent\ntr
 ansi-ent state with a 13 ms rise-time showed a novel actuator (A) domain a
 rrangement that exposes the\nADP-binding site after phosphorylation. Hence
 \, the obtained TR-XSS models determine the relative timing\nof so-far elu
 sive domain rearrangements in a native environment.\n\nhttps://lindico453.
 srv.lu.se/event/125/contributions/773/
LOCATION:Kulturen Auditorium
URL:https://lindico453.srv.lu.se/event/125/contributions/773/
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