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SUMMARY:Neutron research\, Contr. talk 5 - The solution structure of the p
 entameric ligand-gated ion channel GLIC probed by small-angle neutron scat
 tering
DTSTART;VALUE=DATE-TIME:20210510T132000Z
DTEND;VALUE=DATE-TIME:20210510T134000Z
DTSTAMP;VALUE=DATE-TIME:20260528T095157Z
UID:indico-contribution-1071@lindico453.srv.lu.se
DESCRIPTION:Speakers: Marie Lycksell (Stockholm University)\nPentameric li
 gand-gated ion channels undergo subtle conformational cycling to control e
 lectrochemical signal transduction in many kingdoms of life. Several cryst
 al structures have now been reported in this family\, but the functional r
 elevance of such models remains unclear. We used small-angle neutron scatt
 ering (SANS) to probe ambient solution-phase properties of the pH-gated ba
 cterial ion channel GLIC under resting and activating conditions. Data col
 lection was optimized by inline paused-flow size-exclusion chromatography\
 , and exchanging into deuterated detergent to hide the micelle contributio
 n. Resting-state GLIC was the best-fit crystal structure to SANS curves\, 
 with no evidence for divergent mechanisms. Moreover\, enhanced-sampling mo
 lecular dynamics simulations enabled differential modeling in resting vers
 us activating conditions\, with the latter corresponding to an intermediat
 e ensemble of both the extracellular and transmembrane domains. This work 
 demonstrates state-dependent changes in a pentameric ion channel by SANS\,
  an increasingly applicable method for macromolecular characterization as 
 neutron source brilliance increases and inline SEC-SANS set-ups become inc
 reasingly accessible.\n\nhttps://lindico453.srv.lu.se/event/214/contributi
 ons/1071/
LOCATION:
URL:https://lindico453.srv.lu.se/event/214/contributions/1071/
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