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SUMMARY:Contr. talk - Aquaporins – expression\, purification and charact
 erization
DTSTART;VALUE=DATE-TIME:20210525T113500Z
DTEND;VALUE=DATE-TIME:20210525T115500Z
DTSTAMP;VALUE=DATE-TIME:20260722T133153Z
UID:indico-contribution-1130@lindico453.srv.lu.se
DESCRIPTION:Speakers: Kristina Hedfalk ()\nAquaporin water channels facili
 tate the bi-directional flow of water and small\, neutral solutes down an 
 osmotic gradient in all kingdoms of life. Over the last two decades\, the 
 availability of\nhigh-quality protein has underpinned progress in the stru
 ctural and functional characterization of these water channels. In particu
 lar\, recombinant protein technology has guaranteed the supply of aquapori
 n samples that were of sufficient quality and quantity for further study. 
 Here we review the features of successful expression\, purification and ch
 aracterization strategies that have underpinned these successes and that w
 ill drive further breakthroughs in the field. Summarizing\nthe production 
 processes which has resulted in high resolution aquaporin structures revea
 ls that Escherichia coli is a suitable host for prokaryotic isoforms\, whi
 le Pichia pastoris is the most\ncommonly used recombinant host for eukaryo
 tic variants. Generally\, a two-step purification procedure is applied aft
 er solubilization in glucopyranosides and most structures are determined b
 y X-ray following crystallization.\n\nhttps://lindico453.srv.lu.se/event/2
 19/contributions/1130/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1130/
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