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SUMMARY:Keynote talk - An yeast GFP-based platform for functional and stru
 ctures investigation of SLC transporters
DTSTART;VALUE=DATE-TIME:20210525T120000Z
DTEND;VALUE=DATE-TIME:20210525T124000Z
DTSTAMP;VALUE=DATE-TIME:20260722T100015Z
UID:indico-contribution-1131@lindico453.srv.lu.se
DESCRIPTION:Speakers: David Drew (Stockholm University\, Sweden)\nRecombin
 ant expression screening of eukaryotic membrane proteins for functional an
 d structural investigation is still a trial-and-error process. We have fou
 nd that cloning gene-strings by homologuous recombination into a 2u vector
  S. cerevisiae expression vector and then detecting membrane protein expre
 ssion and stability by working with GFP-fusions\, enables many constructs 
 to be rapidly tested. We have found an excellent correlation between the b
 est-behaving membrane proteins in yeast to those produced by transient tra
 nsfection in mammalian HEK293 cells. Here\, I will outline these GFP-based
  methods with a focus on ion and sugar SLC transporters.\n\nhttps://lindic
 o453.srv.lu.se/event/219/contributions/1131/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1131/
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