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SUMMARY:Contr. talk - Production of insect odorant receptors in yeast for 
 structural studies
DTSTART;VALUE=DATE-TIME:20210525T130000Z
DTEND;VALUE=DATE-TIME:20210525T132000Z
DTSTAMP;VALUE=DATE-TIME:20260722T100015Z
UID:indico-contribution-1133@lindico453.srv.lu.se
DESCRIPTION:Speakers: Cassie Sims (Lund University)\nInsect olfactory rece
 ptors (ORs) are proteins involved in olfaction in insects. Insect ORs are 
 7-transmembrane domain proteins that possess a unique structure\, forming 
 a heteromeric complex with a highly conserved co-receptor (ORCO). The hete
 romeric complex constitutes an ion channel\, which opens allowing movement
  of ions upon activation by a ligand. Specificity of ORs is variable\, and
  little is understood about where this specificity arises and how the bind
 ing of an active ligand subsequently opens the ion channel. Despite signif
 icant functional studies of insect ORs\, only two structures\, ORCO\nfrom 
 the fig wasp Apocrypta bakeri\, and a homomeric complex of OR5 from the ju
 mping bristletail\, Machilis hrabei\, have been determined. This structura
 l work relied on a well-established\nexpression system\, HEK293 cells\, wh
 ich are commonly used for functional screening of ORs. However\, HEK cells
  are not an optimal system for protein production for structural analysis.
  In this project\, we begin to develop a more efficient protein production
  system for structural investigation of insect ORORCO\ncomplexes\, focusin
 g on ORs of Lepidoptera and Culicidae (moths and mosquitoes). Initial work
  has focused on the expression of insect OR-ORCO complexes with a fusion e
 GFP in a Pichia pastoris yeast.\n\nhttps://lindico453.srv.lu.se/event/219/
 contributions/1133/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1133/
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