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SUMMARY:Contr. talk  - Production and purification of the K+-dependent P-T
 ype ATPase KdpFABC for crystallization and cryo-EM
DTSTART;VALUE=DATE-TIME:20210525T141000Z
DTEND;VALUE=DATE-TIME:20210525T143000Z
DTSTAMP;VALUE=DATE-TIME:20260722T000810Z
UID:indico-contribution-1135@lindico453.srv.lu.se
DESCRIPTION:Speakers: Charlott Stock (Aarhus University)\nAt very low extr
 acellular K+ concentrations (<100 μM)\, KdpFABC rescues bacterial cells b
 y taking up K+ against an up to 105-fold concentration gradient. To unders
 tand the mechanism of this heterotetrameric complex\, KdpFABC was\npurifie
 d for functional and structural investigations. A new expression system fo
 r KdpFABC was generated\, a 3-step chromatographic purification implemente
 d and protein functionality in different detergents as well as in the pres
 ence of\ninhibitors was tested. The work was performed in the lab of Prof.
  Inga Hänelt at the University of Frankfurt from 2014-2020.\n\nhttps://li
 ndico453.srv.lu.se/event/219/contributions/1135/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1135/
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