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SUMMARY:Contr. talk - Production of Bcl-2 proteins involved in regulation 
 of mitochondrial apoptosis
DTSTART;VALUE=DATE-TIME:20210526T080000Z
DTEND;VALUE=DATE-TIME:20210526T082000Z
DTSTAMP;VALUE=DATE-TIME:20260722T001303Z
UID:indico-contribution-1138@lindico453.srv.lu.se
DESCRIPTION:Speakers: Jörgen Ådén (Umeå University)\nProgrammed cell d
 eath (apoptosis) is essential for human life. In its intrinsic pathway\, t
 he Bcl-2 (B-cell lymphoma 2) protein family regulates cell life and death 
 by controlling permeability of the mitochondrial outer membrane (MOM). How
 ever\, the molecular basis of cell protection by its anti-apoptotic Bcl-2 
 members remains elusive due to the lack of detailed structural insight int
 o their action at the MOM to ensure its integrity. To provide atomic-level
  insight into their functioning\, we will use the founding member of this 
 family\, the human antiapoptotic Bcl-2 protein itself\, whose involvment i
 n p53 regulation and its overexpression\nplays a notorious role in many ca
 ncers and their treatment resistance. However\, for a long time\, obtainin
 g sufficient protein was cumbersome due to its insolubility as a membrane\
 nprotein and low yields. Therefore\, we establishes an expression and puri
 fication protocol [1] to produce routinely mg amounts of the fully functio
 nal full-length human isoform 2 of Bcl-2\n(Bcl-2(2)) which can also be iso
 topically labelled as 15N/13C/2H versions\, ideally suited for neutron and
  NMR studies. The protocol even allows to generate residue specific Bcl-2\
 nmutants and various constructs [2].\n\n1. Aden\, A.U. Mushtaq\, A. Dingel
 dein\, M. Wallgren\, G. Gröbner. A novel recombinant expression and purif
 ication approach for the full-length anti-apoptotic membrane protein Bcl-2
 . Protein expression and purification. 172 (2020) 105628.\n2. A. Ul Mushta
 q\, J. Aden\, T. Sparrman\, M. Hedenstrom\, G. Gröbner. Insight into Func
 tional Membrane Proteins by Solution NMR: The Human Bcl-2 Protein-A Promis
 ing Cancer Drug Target. Molecules 26 (2021) 1467\, 1-14\n\nhttps://lindico
 453.srv.lu.se/event/219/contributions/1138/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1138/
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