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SUMMARY:Keynote talk - Whipping GPCRs into shape for structure determinati
 on
DTSTART;VALUE=DATE-TIME:20210526T083000Z
DTEND;VALUE=DATE-TIME:20210526T091000Z
DTSTAMP;VALUE=DATE-TIME:20260721T210944Z
UID:indico-contribution-1139@lindico453.srv.lu.se
DESCRIPTION:Speakers: Christopher G. Tate (MRC Laboratory of Molecular Bio
 logy\, UK)\nOver the past 15 years we have determined multiple structures 
 of GPCRs by both X-ray crystallography and cryo-EM. For each GPCR we had t
 o overcome the difficulties of overexpression\, solubilisation\, stabilisa
 tion and structure determination\, as you would for any membrane protein. 
 For X-ray crystallography\, extensive protein engineering was required to 
 form well-diffracting crystals\, in parallel with judicious choices of det
 ergent\, ligand and crystallisation format (vapour diffusion or lipidic cu
 bic phase). Different strategies also had to be devised depending on which
  conformational state was required for structure determination. The advent
  of high-resolution structure determination by single particle cryo-EM has
  presented new opportunities for GPCR structural biology\, and structures 
 are now being determined that would have been onerous\, if not impossible\
 , to determine by X-ray crystallography. I will present the key factors fo
 r expression\, purification and structure\ndetermination that have made GP
 CR structures possible.\n\nhttps://lindico453.srv.lu.se/event/219/contribu
 tions/1139/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1139/
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