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SUMMARY:Contr. talk - From expression to purified protein: a P4-ATPase for
  CryoEM studies
DTSTART;VALUE=DATE-TIME:20210526T091000Z
DTEND;VALUE=DATE-TIME:20210526T093000Z
DTSTAMP;VALUE=DATE-TIME:20260722T001303Z
UID:indico-contribution-1140@lindico453.srv.lu.se
DESCRIPTION:Speakers: LineMarie Christiansen (Aarhus University)\nThe Sacc
 haromyces cerevisiae P4-ATPase Neo1p is believed to function as a lipid fl
 ippase\, translocating lipids towards the cytosolic leaflet of both Golgi 
 and endosome membranes. In\norder to screen around both functional and str
 uctural studies to ascertain the exact role and function of Neo1p an effic
 ient high-yield expression and purification protocol is vital. By\noverexp
 ression in S. cerevisiae and purification based on a high-specificity tag 
 we are left with a pure protein sample ideal for both structural and funct
 ional studies. For CryoEM\ndetergent-exchange into LMNG can be easily perf
 ormed\, with some attention to detergent concentration\n\nhttps://lindico4
 53.srv.lu.se/event/219/contributions/1140/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1140/
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