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SUMMARY:Contr. talk - Production of insect odorant receptors in yeast for 
 structural studies
DTSTART;VALUE=DATE-TIME:20210525T130000Z
DTEND;VALUE=DATE-TIME:20210525T132000Z
DTSTAMP;VALUE=DATE-TIME:20260722T045204Z
UID:indico-contribution-183-1133@lindico453.srv.lu.se
DESCRIPTION:Speakers: Cassie Sims (Lund University)\nInsect olfactory rece
 ptors (ORs) are proteins involved in olfaction in insects. Insect ORs are 
 7-transmembrane domain proteins that possess a unique structure\, forming 
 a heteromeric complex with a highly conserved co-receptor (ORCO). The hete
 romeric complex constitutes an ion channel\, which opens allowing movement
  of ions upon activation by a ligand. Specificity of ORs is variable\, and
  little is understood about where this specificity arises and how the bind
 ing of an active ligand subsequently opens the ion channel. Despite signif
 icant functional studies of insect ORs\, only two structures\, ORCO\nfrom 
 the fig wasp Apocrypta bakeri\, and a homomeric complex of OR5 from the ju
 mping bristletail\, Machilis hrabei\, have been determined. This structura
 l work relied on a well-established\nexpression system\, HEK293 cells\, wh
 ich are commonly used for functional screening of ORs. However\, HEK cells
  are not an optimal system for protein production for structural analysis.
  In this project\, we begin to develop a more efficient protein production
  system for structural investigation of insect ORORCO\ncomplexes\, focusin
 g on ORs of Lepidoptera and Culicidae (moths and mosquitoes). Initial work
  has focused on the expression of insect OR-ORCO complexes with a fusion e
 GFP in a Pichia pastoris yeast.\n\nhttps://lindico453.srv.lu.se/event/219/
 contributions/1133/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1133/
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SUMMARY:Contr. talk - Study of anti-cancer effects of TTA-A2 and paclitaxe
 l due to antagonistic interactions with T-type calcium channels
DTSTART;VALUE=DATE-TIME:20210525T124000Z
DTEND;VALUE=DATE-TIME:20210525T130000Z
DTSTAMP;VALUE=DATE-TIME:20260722T045204Z
UID:indico-contribution-183-1132@lindico453.srv.lu.se
DESCRIPTION:Speakers: Vikram Dalal (Washington University in St. Louis)\nS
 tudies have shown that in cancer cells\, there is an increased T-type calc
 ium channel (TTCC) expression compared to healthy cells. Therefore\, the s
 tudies targeting TTCC for cancer therapy\nhave shown many positive outcome
 s. Here\, we have used TTA-A2- a potent TTCC inhibitor as a test drug\, an
 d paclitaxel (PTX)- a tubule-binding anti-cancer agent as a positive contr
 ol. Blocking\nTTCC has shown to overcome resistance in cancer cells toward
 s anti-cancer drugs by reducing calcium influx\, and some studies have sho
 wn that PTX treatment also reduces the intracellular calcium signaling in 
 cells. So\, there is a possibility that PTX might be interacting with calc
 ium channels. Since\, drug-drug interaction can cause severe side-effects\
 , or alter the actions of each other\; we aim to study the interactions am
 ong TTA-A2\, PTX\, and TTCC. Therefore\, in this study we have analyzed th
 e binding of of TTA-A2 and PTX with TTCC. Our results showed that both the
  drugs\, TTA-A2 and PTX\, could interact at the same site of TTCC to form 
 a higher stable complex as compared to the TTCC-native. The result indicat
 ed that sequential treatment could help to overcome the antagonistic inter
 action between the two drugs.\n\nhttps://lindico453.srv.lu.se/event/219/co
 ntributions/1132/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1132/
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SUMMARY:Keynote talk - An yeast GFP-based platform for functional and stru
 ctures investigation of SLC transporters
DTSTART;VALUE=DATE-TIME:20210525T120000Z
DTEND;VALUE=DATE-TIME:20210525T124000Z
DTSTAMP;VALUE=DATE-TIME:20260722T045204Z
UID:indico-contribution-183-1131@lindico453.srv.lu.se
DESCRIPTION:Speakers: David Drew (Stockholm University\, Sweden)\nRecombin
 ant expression screening of eukaryotic membrane proteins for functional an
 d structural investigation is still a trial-and-error process. We have fou
 nd that cloning gene-strings by homologuous recombination into a 2u vector
  S. cerevisiae expression vector and then detecting membrane protein expre
 ssion and stability by working with GFP-fusions\, enables many constructs 
 to be rapidly tested. We have found an excellent correlation between the b
 est-behaving membrane proteins in yeast to those produced by transient tra
 nsfection in mammalian HEK293 cells. Here\, I will outline these GFP-based
  methods with a focus on ion and sugar SLC transporters.\n\nhttps://lindic
 o453.srv.lu.se/event/219/contributions/1131/
LOCATION:
URL:https://lindico453.srv.lu.se/event/219/contributions/1131/
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