BEGIN:VCALENDAR VERSION:2.0 PRODID:-//CERN//INDICO//EN BEGIN:VEVENT SUMMARY:Keynote talk - Models of biological membranes: complex lipid compo sition and membrane proteins DTSTART;VALUE=DATE-TIME:20211208T090000Z DTEND;VALUE=DATE-TIME:20211208T094000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1354@lindico453.srv.lu.se DESCRIPTION:Speakers: Alessandra Luchini ()\nBiological membranes are mai nly composed of lipids and proteins. Unfortunately\, the complex compositi on of biological membranes prevents their direct investigation with biophy sical methods. Therefore\, most physico-chemical and biophysical studies o n biological membranes rely on simple models composed of lipid bilayers in cluding only 1-3 lipid species and in fewer cases membrane proteins. Among all\, supported lipid bilayers represent suitable systems to mimic the li pid component of biological membranes and allow for structural and dynamic investigations by means of several surface sensitive techniques. However\ , loading membrane proteins with controlled orientation in a supported lip id bilayer remains a challenge in this field.[1]\nRecently\, we showed the preparation and characterization of lipid bilayers including either compl ex lipid mixtures\, i.e. lipid extract from the yeast Pichia Pastoris\, or membrane proteins. Supported lipid bilayers with or without membrane prot eins were prepared by a recently developed protocol. The method is based\n on the application of peptide-discs [2]\, a specific kind of nanodiscs whe re the protein belt is composed by self-assembled 18A peptide molecules. T he peptide discs can be adsorbed on the hydrophilic surface of the solid s upport and since the formation of the 18A belt is reversible\, they can be disassembled by rinsing with fresh buffer solution. We showed that this m ethod can successfully lead to the production of supported lipid bilayer w ith biologically relevant lipid compositions [3] and also to the incorpora tion of membrane protein with asymmetric structure\, i.e. composed by one large extramembrane domain (EMD) a transmembrane domain (TMD).[4]\nReferen ces:\n[1] G. Fragneto et al.\, Current Opinion in Colloid & Interface Scie nce\, 2018\, 38\, 108-121.\n[2] S. R. Mitdgaard et al.\, Soft Matter\, 201 4\, 10\, 738-752.\n[3] A. Luchini et al.\, Anlytical Chemistry\, 2020\, 92 \, 1\, 1081-1088.\n[4] A. Luchini et al.\, JCIS\, 2021\, 585\, 376-385.\n\ nhttps://lindico453.srv.lu.se/event/250/contributions/1354/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1354/ END:VEVENT BEGIN:VEVENT SUMMARY:Closing remarks DTSTART;VALUE=DATE-TIME:20211208T111500Z DTEND;VALUE=DATE-TIME:20211208T112000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1357@lindico453.srv.lu.se DESCRIPTION:https://lindico453.srv.lu.se/event/250/contributions/1357/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1357/ END:VEVENT BEGIN:VEVENT SUMMARY:Keynote talk - Unraveling the gating of ligand-gated ion channels through cryo-EM\, SANS\, electrophysiology & molecular simulations DTSTART;VALUE=DATE-TIME:20211208T101000Z DTEND;VALUE=DATE-TIME:20211208T105000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1356@lindico453.srv.lu.se DESCRIPTION:Speakers: Erik Lindahl ()\nhttps://lindico453.srv.lu.se/event/ 250/contributions/1356/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1356/ END:VEVENT BEGIN:VEVENT SUMMARY:Contr. talk 6 - Structural investigation of TSPO translocator comb ining SANS and ab initio simulation DTSTART;VALUE=DATE-TIME:20211208T095000Z DTEND;VALUE=DATE-TIME:20211208T101000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1353@lindico453.srv.lu.se DESCRIPTION:Speakers: Sophie Combet ()\nhttps://lindico453.srv.lu.se/event /250/contributions/1353/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1353/ END:VEVENT BEGIN:VEVENT SUMMARY:Contr. talk 5 - Structural Characterization of a Cholesterol-produ cing Enzyme - Squalene Monooxygenase DTSTART;VALUE=DATE-TIME:20211208T084000Z DTEND;VALUE=DATE-TIME:20211208T090000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1352@lindico453.srv.lu.se DESCRIPTION:Speakers: Emilie Müller ()\nhttps://lindico453.srv.lu.se/even t/250/contributions/1352/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1352/ END:VEVENT BEGIN:VEVENT SUMMARY:Keynote talk - XFEL- and synchrotron-based serial crystallography studies of the membrane-bound proton pump cytochrome c oxidase DTSTART;VALUE=DATE-TIME:20211208T080000Z DTEND;VALUE=DATE-TIME:20211208T084000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1351@lindico453.srv.lu.se DESCRIPTION:Speakers: Gisela Brändén ()\nhttps://lindico453.srv.lu.se/ev ent/250/contributions/1351/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1351/ END:VEVENT BEGIN:VEVENT SUMMARY:Keynote talk - Time-resolved fs crystallography: Discoveries and c hallenges DTSTART;VALUE=DATE-TIME:20211207T150000Z DTEND;VALUE=DATE-TIME:20211207T154000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1349@lindico453.srv.lu.se DESCRIPTION:Speakers: Petra Fromme ()\nhttps://lindico453.srv.lu.se/event/ 250/contributions/1349/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1349/ END:VEVENT BEGIN:VEVENT SUMMARY:Contr. talk 4 - Structural characterization of transpeptidase sort ase enzyme from oral pathogen Streptococcus sanguinis DTSTART;VALUE=DATE-TIME:20211207T144000Z DTEND;VALUE=DATE-TIME:20211207T150000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1347@lindico453.srv.lu.se DESCRIPTION:Speakers: Smita Yadav ()\nhttps://lindico453.srv.lu.se/event/2 50/contributions/1347/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1347/ END:VEVENT BEGIN:VEVENT SUMMARY:Contr. talk 3 - Combination of SAS techniques for modeling deterge nt/IMP interactions DTSTART;VALUE=DATE-TIME:20211207T141000Z DTEND;VALUE=DATE-TIME:20211207T143000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1346@lindico453.srv.lu.se DESCRIPTION:Speakers: Francoise Bonneté ()\nhttps://lindico453.srv.lu.se/ event/250/contributions/1346/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1346/ END:VEVENT BEGIN:VEVENT SUMMARY:Keynote talk - Intestinal peptide and drug uptake – a structure perspective DTSTART;VALUE=DATE-TIME:20211207T131000Z DTEND;VALUE=DATE-TIME:20211207T135000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1345@lindico453.srv.lu.se DESCRIPTION:Speakers: Christian Löw ()\nNutrient uptake across the lipid bilayer is essential for life. Membrane transporters with specialized func tions have evolved to maintain the nutrient homeostasis of cells. Many of those are energized by an electrochemical proton gradient. Prominent membe rs of such ’secondary active transport system’ are the oligopeptide tr ansporters PepT1 and PepT2. Both belong to the Solute Carrier Family 15 (S LC15)\, which is highly conserved among all phyla of life. They are known to play key roles in human diseases and impact the pharmacokinetic profile s of orally administered drug molecules. I will highlight the approaches t hat allowed us to structurally characterize this transporter family. We st udied transporter homologs (from bacteria to humans) by structural (X-ray crystallography\, SAXS\, and cryo-EM)\, biochemical and biophysical approa ches\, and determined the molecular basis for ligand recognition. The tran sporter has been captured in different conformations allowing us to obtain a molecular picture of the transport cycle.\n\nhttps://lindico453.srv.lu. se/event/250/contributions/1345/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1345/ END:VEVENT BEGIN:VEVENT SUMMARY:Contr. talk 2 - Single particle cryo-EM structure of human AQP7 ad opting the formation of dimer of tetramers DTSTART;VALUE=DATE-TIME:20211207T135000Z DTEND;VALUE=DATE-TIME:20211207T141000Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1344@lindico453.srv.lu.se DESCRIPTION:Speakers: Peng Huang ()\nhttps://lindico453.srv.lu.se/event/25 0/contributions/1344/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1344/ END:VEVENT BEGIN:VEVENT SUMMARY:Contr. talk 1 - Design and characterization of a new generation of amphipols for solubilizing and stabilizing membrane proteins DTSTART;VALUE=DATE-TIME:20211207T124500Z DTEND;VALUE=DATE-TIME:20211207T130500Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1343@lindico453.srv.lu.se DESCRIPTION:Speakers: Manuela Zoonens ()\nhttps://lindico453.srv.lu.se/eve nt/250/contributions/1343/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1343/ END:VEVENT BEGIN:VEVENT SUMMARY:Keynote talk - Sample optimisation and characterisation of Membran e proteins DTSTART;VALUE=DATE-TIME:20211207T120500Z DTEND;VALUE=DATE-TIME:20211207T124500Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1342@lindico453.srv.lu.se DESCRIPTION:Speakers: Maria Marta Garcia Alai ()\nhttps://lindico453.srv. lu.se/event/250/contributions/1342/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1342/ END:VEVENT BEGIN:VEVENT SUMMARY:Welcome and purpose of the workshop DTSTART;VALUE=DATE-TIME:20211207T120000Z DTEND;VALUE=DATE-TIME:20211207T120500Z DTSTAMP;VALUE=DATE-TIME:20260530T094624Z UID:indico-contribution-250-1341@lindico453.srv.lu.se DESCRIPTION:https://lindico453.srv.lu.se/event/250/contributions/1341/ LOCATION: URL:https://lindico453.srv.lu.se/event/250/contributions/1341/ END:VEVENT END:VCALENDAR