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SUMMARY:Serial crystallography at the beamline P11\, PETRA III
DTSTART;VALUE=DATE-TIME:20250922T131000Z
DTEND;VALUE=DATE-TIME:20250922T132500Z
DTSTAMP;VALUE=DATE-TIME:20260526T034235Z
UID:indico-contribution-1829@lindico453.srv.lu.se
DESCRIPTION:Speakers: Guillaume Pompidor (DESY)\nP11 at PETRA III (DESY\, 
 Hamburg) is a high-throughput instrument for macromolecular crys-\ntallogr
 aphy [1]. The beamline has tuneable photon energy between 5.5 - 28 keV and
  and beam\nsizes from 200 x 200 μm to 4 x 9 μm2 can be used with a maxim
 um photon flux of 1x1013 ph/s at\n12 keV energy. Equipped with a fast dete
 ctor Eiger2 X 16M as the stationary detector (max 133 Hz)\,\nthis high flu
 x instrument is perfect for serial synchrotron crystallography (SSX).\nFor
  SSX\, the sample delivery is achieved through various types of solid supp
 orts and the TapeDrive\nsetup\, which allows time-resolved room temperatur
 e experiments by the mix-and-diffuse method\n[2\, 3]\, and has been develo
 ped at P11 along with the real-time autoprocessing with CrystFEL [4\,\n5].
  In this method\, the samples are delivered on to a continuously drawn pol
 yimide tape through\na 3D-printed microfluidic nozzle with two channels (
 ø 150 μm each)\, one for the crystal slurry and\nsecond\, for example\, 
 for mixing the crystals with a ligand or adjusting their pH. The mixing ti
 me\ncan be modified by the speed of the tape and the distance of the nozzl
 e from the X-ray focus point\n(delay times of 50 ms – 180 s).\nIn order 
 to mitigate possible radiation damage\, the exposure time per frame can be
  reduced from\n7.5 ms instead of 3.5 ms with a chopper wheel. SSX experime
 nts are currently controlled through\na separate graphical user interface\
 , and online data analysis is available for real-time evaluation\nand inde
 xing via OnDA Monitor [6].\nWe will present the TapeDrive setup used for S
 SX\, the real time data processing implemented as\nwell as several scienti
 fic examples exploiting the capabilities of the setup (time-resolved\, mix
 -and-\ndiffuse experiments\, temperature control…).\n[1] Burkhardt\, A.\
 , Pakendorf\, T.\, Reime\, B.\, Meyer\, J.\, Fischer\, P.\, Stübe\, N.\, 
 Panneerselvam\, S.\,\nLorbeer\, O.\, Stachnik\, K.\, Warmer\, M.\, Rödig\
 , P.\, Göries\, D. & Meents\, A. (2016). Eur. Phys. J. Plus\n131\, 56.\n[
 2] Beyerlein\, K. R.\, Dierksmeyer\, D.\, Mariani\, V.\, Kuhn\, M.\, Sarro
 u\, I.\, Ottaviano\, A.\, Awel\, S.\,\nKnoska\, J.\, Fuglerud\, S.\, Jöns
 son\, O.\, Stern\, S.\, Wiedorn\, M. O.\, Yefanov\, O.\, Adriano\, L.\, Be
 an\, R.\,\nBurkhardt\, A.\, Fischer\, P.\, Heymann\, M.\, Horke\, D. A.\, 
 Jungnickel\, K. E. J.\, Kovaleva\, E.\, Lorbeer\, O.\,\nMetz\, M.\, Meyer\
 , J.\, Morgan\, A.\, Pande\, K.\, Panneerselvam\, S.\, Seuring\, C.\, Tols
 tikova\, A.\, Lieske\,\nJ.\, Aplin\, S.\, Roessle\, M.\, White\, T. A.\, C
 hapman\, H. N.\, Meents\, A. & Oberthuer\, D. (2017). IUCrJ\n4\, 769.\n[3]
  Henkel\, A.\, Maracke\, J.\, Munke\, A.\, Galchenkova\, M.\, Rahmani Mash
 hour\, A.\, Reinke\, P.\, Do-\nmaracky\, M.\, Fleckenstein\, H.\, Hakanpä
 ä\, J.\, Meyer\, J.\, Tolstikova\, A.\, Carnis\, J.\, Middendorf\, P.\,\n
 Gelisio\, L.\, Yefanov\, O.\, Chapman\, H. N. & Oberthür\, D. (2022). Act
 a Cryst. A78\, e560.\n[4] White\, T. A.\, Mariani\, V.\, Brehm\, W.\, Yefa
 nov\, O.\, Barty\, A.\, Beyerlein\, K. R.\, Chervinskii\, F.\,\nGalli\, L.
 \, Gati\, C.\, Nakane\, T.\, Tolstikova\, A.\, Yamashita\, K.\, Yoon\, C. 
 H.\, Diederichs\, K. & Chapman\,\nH. N. (2016). J. Appl. Cryst. 49\, 680.\
 n[5] White\, T. A. Schoof\, T.\, Yakubov\, S.\, Tolstikova\, A.\, Middendo
 rf\, P.\, Karnevskiy\, M.\, Mariani\, V.\,\nHenkel\, A.\, Klopprogge\, B.\
 , Hannappel\, J.\, Oberthür\, D.\, De Gennaro Aquino\, I.\, Egorov\, D.\,
  Munke\,\nA.\, Sprenger\, J.\, Pompidor\, G.\, Taberman\, H.\, Gruzinov\, 
 A.\, Meyer\, J.\, Hakanpää\, J.\, & Gasthuber\,\nG. (2025). IUCrJ 12\, 9
 7.\n[6] Mariani\, V.\, Morgan\, A.\, Yoon\, C. H.\, Lane\, T. J.\, White\,
  T. A.\, O’Grady\, C.\, Kuhn\, M.\, Aplin\, S.\,\nKoglin\, J.\, Barty\, 
 A. & Chapman\, H. N. (2016). J. Appl. Cryst. 49\, 1073.\n\n\nCo-authors:\n
 Alessandra Henkel\, Julia Maracke\, Spyridon Chatziefthymiou\, Alexander G
 rebentsov\, Andrey Gruzinov\, Olga Merkulova\, Philipp Middendorf\, Alexan
 dra Tolstikova\, Thomas White\, Dominik Oberthür\, Johanna Hakanpää\n\n
 https://lindico453.srv.lu.se/event/583/contributions/1829/
LOCATION:LINXS at The Loop
URL:https://lindico453.srv.lu.se/event/583/contributions/1829/
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