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SUMMARY:Reducing data volume with X-ray Laue diffraction
DTSTART;VALUE=DATE-TIME:20250922T163000Z
DTEND;VALUE=DATE-TIME:20250922T164000Z
DTSTAMP;VALUE=DATE-TIME:20260526T135935Z
UID:indico-contribution-1839@lindico453.srv.lu.se
DESCRIPTION:Speakers: Kah Chee Pow (Spallation Neutron Source Science Cent
 er)\nThe X-ray Laue diffraction captures the crystal diffraction from whit
 e beam and can contain significant amount of structural information in com
 parison to monochromatic diffraction. In time-resolved study\, the serial 
 femtosecond crystallography (SFX) and serial synchrotron crystallography (
 SSX) are the mainstream approaches with the caveat of generating large dat
 a volume. Laue diffraction has great potential in mitigating the data volu
 me challenge and has recently regain the interest by the community. Here\,
  we share the preliminary works conducted at BL03HB Laue Micro-diffraction
  Beamline at Shanghai Synchrotron Radiation Facility (SSRF) to compare wit
 h conventional method. We crystallized an apo stilbene synthase (STS) with
  inherent loop conformation duality and collected diffraction data via (i)
  single-crystal\, cryogenic\, rotation approach with monochromatic beam\, 
 (ii) single-crystal\, ambient\, helical approach with white beam\, and (ii
 i) multi-crystal\, ambient\, single-frame approach with white beam. Compar
 e to monochromatic diffraction\, Laue diffraction dataset show increased c
 ontent of structural information per image but with poor statistical metri
 cs upon merging. Nevertheless\, the solved structure from Laue diffraction
  dataset can resolve the duality of the loop conformation and reveal the l
 oop conformational preference at productive state at ambient temperature. 
 In short\, Laue diffraction is a promising approach to explore in mitigati
 ng the data volume challenge.\n\nAuthor: Kah Chee Pow\nCo-author: Quan Hao
 \n\nhttps://lindico453.srv.lu.se/event/583/contributions/1839/
LOCATION:LINXS at The Loop
URL:https://lindico453.srv.lu.se/event/583/contributions/1839/
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