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SUMMARY:Time and dose resolved crystallography to control and capture redo
 x states in heme peroxidases
DTSTART;VALUE=DATE-TIME:20250922T170000Z
DTEND;VALUE=DATE-TIME:20250922T171000Z
DTSTAMP;VALUE=DATE-TIME:20260526T203233Z
UID:indico-contribution-1841@lindico453.srv.lu.se
DESCRIPTION:Speakers: Michael Hough (Diamond Light Source)\nMetalloenzymes
  containing heme centres catalyse a wide range of reactions critical to li
 fe. Understanding the structure and electronic states of the heme centre a
 cross multiple functionally relevant states is essential to understand mec
 hanism. I will describe work using time resolved serial crystallography as
  well as the use of X-ray dose for the manipulation of heme iron oxidation
  states in dye decolourising peroxidases [1] using multiple\, complementar
 y\, serial crystallography and single-crystal spectroscopic approaches.\n\
 nFixed target drop-on-chip\, tape drive droplet on demand and correlated s
 pectroscopies allow the formation of high valent Fe(IV) states to be chara
 cterised. X-ray Pump Probe serial femtosecond crystallography (SFX) togeth
 er with dose-resolved serial synchrotron crystallography (SSX) allowed the
  peroxidases to be driven between multiple iron oxidation states that can 
 be spectroscopically validated. Intriguingly\, the formation and dose resp
 onse of the Fe(IV)-O state is highly variable between the chemically ident
 ical heme groups of the homo-oligomeric proteins highlighting the importan
 ce of understanding the effect of the crystalline lattice on observed chan
 ges in time- and dose-resolved crystallography experiments.\n\nLucic\, M. 
 et al (2021) Aspartate or arginine? Validated redox state X-ray structures
  elucidate mechanistic subtleties of FeIV = O formation in bacterial d
 ye-decolorizing peroxidases. JBIC 27 (7)\, 743-761.\n\n\nCo-authors: Robin
  Owen\, Jonathan Worrall\, Marina Rozman\, Lewis Williams\, Danny Axford\,
  Allen Orville\, Pierre Aller\, Jan Kern\, Jos Kamps\n\nhttps://lindico453
 .srv.lu.se/event/583/contributions/1841/
LOCATION:LINXS at The Loop
URL:https://lindico453.srv.lu.se/event/583/contributions/1841/
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