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SUMMARY:Time-resolved X-ray crystallography on membrane proteins: Watching
  ions moving in time and space
DTSTART;VALUE=DATE-TIME:20250923T070000Z
DTEND;VALUE=DATE-TIME:20250923T073000Z
DTSTAMP;VALUE=DATE-TIME:20260526T203229Z
UID:indico-contribution-1845@lindico453.srv.lu.se
DESCRIPTION:Speakers: Przemyslaw Nogly (Jagiellonian University in Krakow)
 \nP. Nogly\nDioscuri Centre for Structural Dynamics of Receptors\, Faculty
  of Biochemistry\, Biophysics and Biotechnology\, Jagiellonian University\
 , Kraków\, Poland\; przemyslaw.nogly@uj.edu.pl \n\nChloride transport is 
 an essential process maintaining ion balance across cell membranes\, cell 
 growth\, and neuronal action potentials. However\, the molecular mechanism
  of the transport remains elusive. Among chloride transporters\, light-dri
 ven rhodopsins have gained attention as optogenetic tools to manipulate ne
 uronal signaling. We combined time-resolved serial crystallography at XFEL
  and synchrotron to provide a comprehensive view of chloride-pumping rhodo
 psin's structural dynamics and molecular mechanism throughout the transpor
 t cycle from 10 ps to 50 ms [1]. We traced transient anion binding sites\,
  obtained evidence for the mechanism of light energy utilization in transp
 ort\, and identified steric and electrostatic molecular gates ensuring uni
 directional transport. These structural insights provided the basis for mu
 tagenesis and functional study of the mechanistic features enabling finely
  controlled chloride transport across the cell membrane.\nFurthermore\, ou
 r recent study of a distinct photoreceptor\, Light-Oxygen-Voltage (LOV) do
 main\, will be introduced. The first insights into the structural dynamics
  of LOV photoactivation will be presented\, providing the basis for propos
 ing a molecular mechanism of a covalent thioether bond formation between a
  flavin mononucleotide cofactor and a reactive cysteine\, Cys57 (unpublish
 ed).\n\n[1] Mous\, S. et al. Science 375 (2022) 845\n\nhttps://lindico453.
 srv.lu.se/event/583/contributions/1845/
LOCATION:LINXS at The Loop
URL:https://lindico453.srv.lu.se/event/583/contributions/1845/
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