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SUMMARY:Investigating enzyme mechanisms by multidimensional crystallograph
 y
DTSTART;VALUE=DATE-TIME:20250924T070000Z
DTEND;VALUE=DATE-TIME:20250924T073000Z
DTSTAMP;VALUE=DATE-TIME:20260525T195341Z
UID:indico-contribution-1856@lindico453.srv.lu.se
DESCRIPTION:Speakers: Pedram Mehrabi (University of Hamburg)\nFunctional c
 haracterization of proteins requires linking structure and dynamics\, but 
 traditional X-ray crystallography provides only static snapshots. Serial t
 ime-resolved crystallography enables direct visualization of structural ch
 anges over time\, including internal motions and solvent interactions. We 
 developed fixed-target approaches such as “Hit And REturn” (HARE) and 
 reaction initiation strategies using piezo droplet injectors. The “Liqui
 d Application Method for time-resolved Analysis” (LAMA) further broadens
  applicability to systems not triggered by light. In addition\, environmen
 tal control allows temperature variation from 7 °C to 70 °C\, enabling m
 ulti-dimensional experiments. These advances permit direct observation of 
 ligand binding\, intermediates\, and conformational changes\, as demonstra
 ted by tracking glucose-to-fructose conversion in Xylose isomerase across 
 both temperature and time. Together\, these methods expand the toolkit for
  time-resolved crystallography\, opening the way to mechanistic insights i
 nto enzyme dynamics\, allostery\, and solvent networks.\n\nhttps://lindico
 453.srv.lu.se/event/583/contributions/1856/
LOCATION:LINXS at The Loop
URL:https://lindico453.srv.lu.se/event/583/contributions/1856/
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