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SUMMARY:Serial Microsecond Crystallography with the ESRF Extremely Brillia
 nt Source
DTSTART;VALUE=DATE-TIME:20250924T091500Z
DTEND;VALUE=DATE-TIME:20250924T094500Z
DTSTAMP;VALUE=DATE-TIME:20260525T195546Z
UID:indico-contribution-1860@lindico453.srv.lu.se
DESCRIPTION:Speakers: Daniele de Sanctis (ESRF - The European Synchrotron)
 \nTime-resolved serial crystallography (TR-SX) is a leading technique for 
 capturing biological processes as molecular movies on extremely fast times
 cales—fulfilling a long-standing goal in structural biology. The method 
 involves delivering microcrystals into a powerful\, pulsed X-ray beam to c
 ollect individual diffraction patterns from each crystal. By compiling tho
 usands of these patterns\, researchers can reconstruct an electron density
  map. This technique\, known as serial crystallography\, allows for the ob
 servation of structural changes. When ultrashort X-ray pulses are used\, S
 X can track time-dependent conformational changes\, enabling scientists to
  visualize proteins in motion. TR-SX experiments are typically synchronize
 d with a specific stimulus that initiates the biological activity under in
 vestigation. The advent of diffraction limited storage rings - the so-call
 ed 4th generation synchrotrons - have permitted the conception and built i
 nstruments that overcomes the limits of traditional microfocus beamlines. 
 These new beamlines aim to exploit X-ray pulses down to microsecond time r
 esolution\, becoming an invaluable tool for room temperature and time-reso
 lved studies that complements the capabilities of Free Electron Laser sour
 ces. ID29 at the European Synchrotron Radiation Facility is one of the fir
 st examples of this new generation of beamlines (Orlans et al. 2025). The 
 unique combination of microsecond exposure times\, advanced beam propertie
 s\, and a flexible sample environment enables the collection of high-quali
 ty\, complete data—even from exceptionally small amounts of crystalline 
 material. This is applied in combination with external stimuli to activate
  or induce structural changes that could be observed in the microsecond ti
 me regime. This approach is particularly successful for the study of enzym
 atic reaction or ligand binding\, thus prominently interesting for the who
 le structural biology community\, while future developments will be crucia
 l to strengthen the application of the methods to structural based drug de
 sign.\n\nOrlans\, J. (2025). Advancing macromolecular structure determinat
 ion with microsecond X-ray pulses at a 4th generation synchrotron. Communi
 cations Chemistry\, 8(1)\, 1–12. https://doi.org/10.1038/s42004-024-0140
 4-y\n\nhttps://lindico453.srv.lu.se/event/583/contributions/1860/
LOCATION:LINXS at The Loop
URL:https://lindico453.srv.lu.se/event/583/contributions/1860/
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